Table 2

Analysis of fluorescent and non-fluorescent mutants carrying single amino acid substitutions

WT aa

AS

Hydrophobic aa

Hydrophilic aa

Acid

Basic

Stop


W

F

P

M

L

I

V

A

G

N

Q

S

T

C

Y

D

E

R

K

H

X


F131

B

1

1

1

2*

1

1

1

1

1

K132

E

1

1

1

1

1

2

E133

E

1

2

1

3

1

1

1

1

1

3

D134

E

1

1

2

2

2

G135

B

    1

1*

1*

1

1*

    1

    1

N136

E

1

4

1

1

4

4

1

2

1

1

1

I137

B

4

2

1

1*

1

2

2

1

L138

B

1

2

2

1

1*

    1

1

G139

E

1*

2

1

1

1

2

2

1

1

2

1

H140

E

1

1*

1

1

2

2

K141

E

2*

    1

1

1

1

1

1

L142

B

1

1

1

2

1

1

1


Mutants displaying a green-fluorescent phenotype to the naked eye are shown in bold numbers. Mutants displaying a white phenotype are shown in italic numbers. Mutants displaying a pale green-fluorescent phenotype are shown in underlined numbers. Those mutants labeled with asterisk turned pale green-fluorescent after prolonged growing at room temperature. The number represents the number of times that each mutant was found. In this table, empty spaces represent wild-type amino acids. B means buried amino acid; E, solvent exposed amino acid. During screening of fluorescent mutants, seven clones corresponded to wt sgGFP generated with the mixtures of degenerated oligonucleotides.

Flores-Ramírez et al. BMC Chemical Biology 2007 7:1   doi:10.1186/1472-6769-7-1

Open Data