Table 2

Analysis of fluorescent and non-fluorescent mutants carrying single amino acid substitutions
WT aa
 AS
 Hydrophobic aa
 Hydrophilic aa
 Acid
 Basic
 Stop




W
 F
 P
 M
 L
 I
 V
 A
 G
 N
 Q
 S
 T
 C
 Y
 D
 E
 R
 K
 H
 X
F131
 B

1
1
 1
2*

1
1

1

1
 1
K132
 E
 1



1
1
 1






1
 2


E133
 E

1
2
1
 3
 1
 1
1
 1

1
3


D134
 E
1







1
 2
 2



2


G135
 B



1
 1*
 1*
1

1*
1
 1





N136
 E
1
 4
 1
 1
4
 4

1
2



1
 1
 1
I137
 B



4
2
1

1*


1
 2
 2

1
L138
 B
1
2
 2
 1

1*



1


1


G139
 E

1*
2

1
 1
 1
 2
 2
 1
 1



2
 1
H140
 E

1

1*


1



1

2
 2

K141
 E

2*
 1


1
1

1



1
1
L142
 B
 1



1
 1
2
 1
1


1




Mutants displaying a green-fluorescent phenotype to the naked eye are shown in bold numbers. Mutants displaying a white phenotype are shown in italic numbers. Mutants displaying a pale green-fluorescent phenotype are shown in underlined numbers. Those mutants labeled with asterisk turned pale green-fluorescent after prolonged growing at room temperature. The number represents the number of times that each mutant was found. In this table, empty spaces represent wild-type amino acids. B means buried amino acid; E, solvent exposed amino acid. During screening of fluorescent mutants, seven clones corresponded to wt sgGFP generated with the mixtures of degenerated oligonucleotides.

Flores-Ramírez et al. BMC Chemical Biology 2007 7:1   doi:10.1186/1472-6769-7-1