Experimental verification by in vitro biotinylated incednine pull-down assay. Lysates from Ms-1 overexpressing Bcl-xL were incubated with biotin (Biotin) or biotinylated incednine (BI) and avidin beads for 3 h. The beads were washed, and co-precipitated proteins were eluted with 2 mM biotin. The eluted proteins were subjected to western blotting using the indicated antibodies. “Positive candidates” means proteins which were predicted to bind to incednine, and “Negative candidates” means proteins which were predicted not to bind to incednine.
Kobayashi et al. BMC Chemical Biology 2012 12:2 doi:10.1186/1472-6769-12-2