Table 2

Intracellular micromolar concentrations of NAD+ metabolites and related nucleosides and nucleotides as a function of medium components

Metabolite

SDC-NA

SDC

YPD

YPD0.5

YPD0.2


Nam

1.5 ± 0.2*†

31.6 ± 3.0

35.0 ± 3.2

29.7 ± 0.3

51.9 ± 17.9

NA

<0.5

<0.5

<0.5

<0.5

<0.5

Cyt

1.8 ± 0.2†

1.5 ± 0.2†

69.5 ± 11.5

42.2 ± 9.1

63.4 ± 13.5

Urd

5.3 ± 1.4†

13.0 ± 3.0†

139.7 ± 0.6

141.7 ± 0.2

128.7 ± 7.0

NR

2.1 ± 0.3*†

4.6 ± 0.9†

9.3 ± 0.4

7.9 ± 1.5

7.1 ± 1.4

NAR

0.2 ± 0.0*†

8.1 ± 1.3

12.5 ± 0.2

8.3 ± 1.7

8.4 ± 0.7†

Ino

21.8 ± 1.7*†

6.0 ± 0.4†

54.9 ± 2.6

38.5 ± 3.3†

31.8 ± 3.3†

CMP

31.5 ± 1.5

39.7 ± 9.1

146.4 ± 16.7

134.8 ± 45.1

165.3 ± 38.3

UMP

63.0 ± 7.0*†

145.4 ± 27.9

179.6 ± 2.7

238.0 ± 20.9

236.6 ± 23.2

NMN

19.0 ± 1.9*†

67.3 ± 13.0

62.1 ± 13.7

60.0 ± 14.8

72.4 ± 21.2

NaMN

2.9 ± 0.2*†

28.0 ± 4.5

11.6 ± 2.0

18.4 ± 4.3

21.1 ± 3.7

IMP

8.4 ± 0.3

7.1 ± 1.8

16.8 ± 6.3

16.1 ± 1.5

16.1 ± 1.5

NAD+

277.2 ± 26.7*

513.9 ± 51.1

438.5 ± 66.7

308.9 ± 33.8

339.5 ± 11.7

NaAD

<0.1

<0.1†

3.8 ± 0.3

<0.1†

<0.1†

NADH

125.8 ± 13.6*†

207.3 ± 17.1

263.0 ± 55.3

101.2 ± 24.6

152.0 ± 12.7

NADP

31.2 ± 1.4

31.1 ± 3.5

46.9 ± 10.2

42.9 ± 7.2

52.5 ± 2.1

NADPH

0.3 ± 0.1*

3.1 ± 0.6

0.7 ± 0.2

1.3 ± 0.1

3.0 ± 0.2†‡


* indicates a significant difference when compared to cells grown in SDC. † indicates a significant difference when compared to cells grown in YPD. ‡ indicates a significant difference between cells grown in YPD 0.5 and YPD 0.2% glucose.

Evans et al. BMC Chemical Biology 2010 10:2   doi:10.1186/1472-6769-10-2

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