Table 1 |
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|
LC-MS/MS SRM parameters and sensitivity details for NAD+ metabolites and related nucleosides and nucleotides |
||||||||
|
Metabolite |
Parent mass |
Product mass |
Cone voltage |
Collision Energy |
RT (min) |
LOQ (pmol) |
R2 |
RSD (%) |
|
|
||||||||
|
Nam |
123 |
80 |
35 |
20 |
3.68 |
0.45 |
0.996 |
7 |
|
NA |
124 |
80 |
35 |
20 |
7.15 |
1.20 |
0.999 |
12 |
|
Cyt |
244 |
112 |
35 |
10 |
4.52 |
0.10 |
0.995 |
10 |
|
Urd |
245 |
113 |
90 |
10 |
4.56 |
1.20 |
0.978 |
10 |
|
NR |
255 |
123 |
50 |
10 |
4.07 |
0.20 |
0.992 |
7 |
|
NAR |
256 |
124 |
35 |
10 |
4.80 |
0.06 |
0.984 |
5 |
|
Ino |
269 |
137 |
80 |
10 |
6.31 |
0.07 |
0.993 |
3 |
|
CMP |
324 |
112 |
35 |
10 |
12.05 |
0.68 |
0.999 |
6 |
|
UMP |
325 |
97 |
90 |
15 |
12.22 |
0.21 |
0.989 |
8 |
|
NMN |
335 |
123 |
35 |
10 |
9.04 |
0.50 |
0.988 |
9 |
|
QA |
166 (-) |
122 |
35 |
10 |
12.34 |
9 |
||
|
NaMN |
336 |
124 |
35 |
10 |
11.66 |
0.18 |
0.999 |
5 |
|
IMP |
349 |
137 |
80 |
10 |
13.59 |
0.13 |
0.997 |
5 |
|
NAD+ |
664 |
542 |
35 |
18 |
8.69 |
0.17 |
0.996 |
8 |
|
NaAD |
665 |
542 |
35 |
20 |
11.59 |
0.26 |
0.987 |
8 |
|
NADH |
666 |
649 |
35 |
20 |
11.77 |
0.06 |
0.991 |
16 |
|
NADP |
744 |
604 |
35 |
25 |
14.01 |
0.87 |
0.995 |
9 |
|
NADPH |
746 |
729 |
35 |
20 |
16.18 |
0.17 |
0.994 |
8 |
|
|
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|
Evans et al. BMC Chemical Biology 2010 10:2 doi:10.1186/1472-6769-10-2 |
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