Non-cancer cells undergo mitotic slippage without activating apoptotic pathways while cancer cells execute apoptosis after DZ treatment. Cells were treated with 10 nM of DZ for 38 hours in the following experiments unless where indicated. 100 nM of VBL and 1 μM of TXL were used as controls. RPE-hTERT cells were used in (a-c). a) cas-inh: Caspase inhibitors (caspase-3/7, -8, and -9 inhibitors). Results are averages of three independent experiments. b) Upper panel: cytochrome C staining. Lower panel: MitoTracker staining. c) Immunoblot analysis of PARP. Upper band: full-length PARP. Lower band: cleaved product of PARP. d) Immunoblot analysis of PARP cleavage in cancer cells after DZ treatment. un: untreated.
Xu et al. BMC Chemical Biology 2010 10:1 doi:10.1186/1472-6769-10-1