Fluorescence microscopy. CHO cells expressing the rSPR were stained with (A) Alexa 488-SP, (B) BODIPY Fl-SP, (C) fluorescein-SP, (D) Oregon Green 488-SP, (E) tetramethylrhodamine-SP and (F) PBS in the absence of a fluorophore. The intensity to area ratio of Alexa 488-SP was significantly less than the other green fluorophores. However a significant difference was not seen between BODIPY Fl-SP, fluorescein-SP or Oregon Green 488-SP.
Bennett and Simmons BMC Chemical Biology 2001 1:1 doi:10.1186/1472-6769-1-1