Table 3

Plant germplasm.

Line

Experiments

Transgenic?

Generation

Parental Lines

Purpose


Brassica napus (BN)

Both

no

n/a

n/a

crop: parental control

Brassica rapa (BR)

Both

no

n/a

n/a

weed: parental control

GT1 hybrid

Both

yes: mGFP5er and Bt cry1Ac

mixed: BC1/F2

BR and transgenic BN

to study event-specific genetic load

GT5 hybrid

Both

yes: mGFP5er and Bt cry1Ac

mixed: BC1/F2

BR and transgenic BN

to study event-specific genetic load

GT9 hybrid

Both

yes: mGFP5er and Bt cry1Ac

mixed: BC1/F2

BR and transgenic BN

to study event-specific genetic load

TM homozygous [TM(H)]

TM only

yes: Δgai and ahas

T2

BN

TM parental control

TM backcross [TM(B)]

TM only

yes: Δgai and ahas

mixed: BC1/F2

BR and transgenic BN

To study transgene persistence in a backcrossed population


Summary of line designations, generations and parental lines for the seven plant germplasm types utilized in this study. The transgene nomenclature is as follows: mGFP5er (gene coding for green fluorescent protein); Bt cry1Ac (Bacillus thuringiensis insecticidal toxin gene); Δgai (gibberellic acid insensitive gene); ahas (acetolactate synthase-herbicide resistance gene).

Rose et al. BMC Biotechnology 2009 9:93   doi:10.1186/1472-6750-9-93

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