Table 3 |
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|
Plant germplasm. |
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|
Line |
Experiments |
Transgenic? |
Generation |
Parental Lines |
Purpose |
|
|
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|
Brassica napus (BN) |
Both |
no |
n/a |
n/a |
crop: parental control |
|
Brassica rapa (BR) |
Both |
no |
n/a |
n/a |
weed: parental control |
|
GT1 hybrid |
Both |
yes: mGFP5er and Bt cry1Ac |
mixed: BC1/F2 |
BR and transgenic BN |
to study event-specific genetic load |
|
GT5 hybrid |
Both |
yes: mGFP5er and Bt cry1Ac |
mixed: BC1/F2 |
BR and transgenic BN |
to study event-specific genetic load |
|
GT9 hybrid |
Both |
yes: mGFP5er and Bt cry1Ac |
mixed: BC1/F2 |
BR and transgenic BN |
to study event-specific genetic load |
|
TM homozygous [TM(H)] |
TM only |
yes: Δgai and ahas |
T2 |
BN |
TM parental control |
|
TM backcross [TM(B)] |
TM only |
yes: Δgai and ahas |
mixed: BC1/F2 |
BR and transgenic BN |
To study transgene persistence in a backcrossed population |
|
|
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|
Summary of line designations, generations and parental lines for the seven plant germplasm types utilized in this study. The transgene nomenclature is as follows: mGFP5er (gene coding for green fluorescent protein); Bt cry1Ac (Bacillus thuringiensis insecticidal toxin gene); Δgai (gibberellic acid insensitive gene); ahas (acetolactate synthase-herbicide resistance gene). |
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|
Rose et al. BMC Biotechnology 2009 9:93 doi:10.1186/1472-6750-9-93 |
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