Methodology article
High quality protein microarray using in situ protein purification
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* Corresponding author: Scott N Peterson scottp@jcvi.org
Pathogen Functional Genomics Resource Center, J. Craig Venter Institute, 9704 Medical Center Drive, Rockville, Maryland 20850, USA
BMC Biotechnology 2009, 9:72 doi:10.1186/1472-6750-9-72
Published: 23 August 2009Additional files
Additional file 1:
Immunoassay data were obtained from an in situ protein microarray with 90 S. pneumoniae proteins. The S-tag assay scores are correlated with the quantities of recombinant proteins in the soluble fraction after cell lysis, therefore the scores represent the solubility of the recombinant proteins (Kwon et al., 2007). The values of H(FHis-tag) represent fluorescence intensities from a labeled anti-His-tag antibody. Values of E(FE. coli) and S(FSp) were obtained from fluorophore-labeled secondary antibodies, following binding of anti-E. coli and anti-S. pneumoniae antibodies, respectively. Values of S1(F27) and S0(F48) result from assays with a human patient antiserum and a healthy human antiserum, respectively.
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