|
Forward (-F) and reverse (-R) primers used for point mutations in α ENaC (a-), and insertion of a 27 nt segment coding for a 9 residue epitope tag in β ENaC (b+) cDNA. |
||
| Primer |
Sequence* |
Length (nt) |
|
|
||
| a-R333K-F |
ctgtccctgatgctgAAGgcagagcagaatgacttc |
36 |
| a-R333K-R |
gaagtcattctgctctgcCTTcagcatcagggacag |
36 |
| a-D338A-F |
cgcgcagagcagaatgCcttcattcccctgctg |
33 |
| a-D338A-R |
cagcaggggaatgaagGcattctgctctgcgcg |
33 |
| a-R350A-F |
cacagtgactggggccGCggtaatggtgcacggg |
34 |
| a-R350A-R |
cccgtgcaccattaccGCggccccagtcactgtg |
34 |
| a-E358D-F |
gtgcacgggcaggatgaTcctgcctttatggatg |
34 |
| a-E358D-R |
catccataaaggcaggAtcatcctgcccgtgcac |
34 |
| a-K474R-F |
ctgggctgtttcaccaGgtgccggaagccatgc |
33 |
| a-K474R-R |
gcatggcttccggcacCtggtgaaacagcccag |
33 |
| a-R476K-F |
ctgtttcaccaagtgcAAgaagccatgcagcgtg |
34 |
| a-R476K-R |
cacgctgcatggcttcTTgcacttggtgaaacag |
34 |
| b+HA-F |
gccattgccaccaggTACCCATACGACGTCCCAGACTACGCTaacctgaacttctcc |
57 |
| b+HA-R |
ggagaagttcaggttAGCGTAGTCTGGGACGTCGTATGGGTAcctggtggcattggc |
57 |
|
* Mutated or inserted nucleotides are written in upper case characters. | ||
Edelheit et al. BMC Biotechnology 2009 9:61 doi:10.1186/1472-6750-9-61 |
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