Table 1

Extent of primary amine acetylation as determined by ¹H NMR spectroscopy.

Sample

(-CH₃/-CH₂-) ratio

Number of acetyl groups added

Percent of primary amine acetylation

Ac₂₀

0.041

27

21.1

Ac₄₀

0.075

50

39.1

Ac₆₀

0.115

76

59.4

Ac₈₄

0.16

107

83.6

Waite et al. BMC Biotechnology 2009 9:38   doi:10.1186/1472-6750-9-38