Table 1

FLIM measurements for the TagGFP and TagGFP-TagRFP pair

Construct

n1

τφ [ns]2

τM [ns]3

φ [%]4

M [%]4


TagGFP

20

2.55 ± 0.05

2.64 ± 0.04

-

-

TagGFP-TagRFP

23

1.57 ± 0.07

1.98 ± 0.07

38

24


1n number of cells from which the lifetime was calculated

2τφ average phase lifetime ± standard deviation

3τM average modulation lifetime ± standard deviation

4E average FRET efficiency calculated from τφ or τM

Shcherbo et al. BMC Biotechnology 2009 9:24   doi:10.1186/1472-6750-9-24

Open Data