Effect of total RNA degradation on miQPCR data. Comparison of changes in relative expression for 7 selected miRNAs (Let-7b, Let-7c, Let-7d, miR-122, miR-16, miR-192 and miR-194) and RNU6 in total RNA extracted from frozen liver. For individual miRNAs the crossing threshold (Ct) value across the seven different miRNAs is shown. Data are shown as average of three independent miQPCR runs ± standard deviation.
Ibberson et al. BMC Biotechnology 2009 9:102 doi:10.1186/1472-6750-9-102