Analysis of miRNA degradation by Northern blot analysis. To evaluate how degradation of total RNA affects miRNA integrity the liver specific miR-122 was selected. The top panel shows ethidium bromide stained 5S RNA as a loading control. In the bottom panel background-corrected signal intensities of the northern blot are calculated by ImageJ. The signal corresponding to the miRNA with the highest signal intensity (T0) was set to 100%, and signals for the other samples were calculated as a percentage of it. The position of the mature miR-122 is indicated (middle panel).
Ibberson et al. BMC Biotechnology 2009 9:102 doi:10.1186/1472-6750-9-102