Table 1

Primers sequences for PCR amplification of DNA encoding the proteins examined here.

Fragments

Primers

Sequence (5' to 3')

Size (bp)


Tat

Tat F

ggg atg gag aca ccc ttg aag

150

Tat R

ggg atg ata gca aca cct ctt

Rev

Rev F

ggg tca gca gat ccc tat cca

243

Rev R

ggg cgg act ctt tgc aac gtc

Nef1

Nef1 F

ggg atg ggt ggc gct att tcc

642

Nef1 R

ggg ctt cca tgc cag tac ctc

gvpC

gvpC F

ctc ctg ctg tga ttc tgc ga

1,070

gvpC R

cat cct cac cgt act cgt cg

gvpC – Kpn I

cta tgg cca cga gat cac g

350


DNA sequences required to generate recombinant GvpC and demonstrate the successful insertion and retention of test SIV DNA sequences utilized PCR amplification and the forward (F) and reverse(R) primers listed above.

Sremac and Stuart BMC Biotechnology 2008 8:9   doi:10.1186/1472-6750-8-9

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