|
Oligonucleotide primers used in this study |
|
| Primer name |
Sequence 5'-3' |
|
|
|
| Tc-5'-PmlNco |
CACGTGCCATGGTTCTCATGTTTGACAGCTTATC |
| Tc-3'-PmlNsi |
CACGTGAT GCATGGACTTCCGCGTTTCCAG |
| SpR-5'-HpaBspNhe |
ATCGTTAACTCATGAGCTAGCCGTTCGTGAATACATGTTATAATA |
| SpR-3'-Hpa |
ATCGTTAACTTATTTGCCGACTACCTTGGTGATCT |
| GFP-mu-5'-Bam |
ATCGGATCCAAAACAATGGCTAGTAAAGGAGAAGAACTTTTCACTGGAGTTGTCCC |
| GFP-mu-3'-NotAvrBgl |
GATATCGCGGCCGCCCTAGGTAGATCTTCCACCTCCACCTTTGTATAGTTCATCCATGCCATGTGTA |
| ABI3-5'-CDS-Rescue-3'-Test |
CTCCTCCATGGTCATCTAACC |
| ABI3-3'-CDS-Rescue-5'-Test |
GTACCCGAGTGTGCCACTTC |
| PAP85-Prom-Rescue-3'-Test |
ACTTCCTAGGGTTGCTACTG |
| PAP85-Term-Rescue-5'-Test |
ACCGGATGGTTTGGT TATGG |
| ABI3-L1-Rescue-5'-CDS1 |
agatctccggcgttggccgccacatgcaagcttttcatcgttgaagtggaCAAATACGCATACTGTTATCTGGCT |
| ABI3-L2-Rescue-3'-CDS1 |
tattacatatgtggctcatttcttgagtcgagcttacatatcgtttataaAACCTGATGTTCTGGGGAATATAG |
| ENTRY-L1 |
TTAGTTAGTTACTTAAGCTCGG |
| ENTRY-L2 |
TGAGACACGGGCCAGAGC |
| PAP85-Rescue-5'-Prom2 |
ttcctaccaccatgaccaaacttggaatcccgaaactccctcttcttgttTTGTCGTTTCCCGCCTTCAG |
| PAP85-Rescue-3'-Term2 |
atagctcgtattttttagcttatgtctaaaatacccctgagatcatgtggAACGTTCTCGAGGGGATCTTCTG |
| pCB-RB |
CTGTGGTTGGCATGCACATA |
| tCUP2-Out |
CGAGGTTAGACCTTCAGGAAATA |
| PAP85-Integ-Dest-5'3 |
acccattattgtcctcaaaaacaaacacatcaacaaaacaacaagaaaaaGATATCACAAGTTTGTACAAAAAAGC |
| PAP85-Integ-Dest-3'3 |
ttaaactcttaaactctttcctttcttaggactgtaaaaaagcattttcaGATATCACCACTTTGTACAAGAAAGC |
| PAP85-Dest-Integ-5'-Test |
TGATGCGTACGAACACTC |
| Cm-5'-AS |
ACGGTGGTATATCCAGTGATT |
| PAP85-Dest-Integ-3'-Test |
GTTACATGCAGAGCG TTACC |
| C1 |
TTATACGCAAGGCGACAAGG |
| PAP85-S1 |
GCAGGGATCCT TGTTCCTTC |
|
1Upper-case letters correspond to unique recognition sequences in pJM1 and lower-case letters correspond to 50 bp regions homologous to ABI3. 2Upper-case letters correspond to unique recognition sequences in pWY109 and pWY107 and lower-case letters correspond to 50 bp regions homologous to PAP85. 3Upper-case letters correspond to unique recognition sequences in the RfA-Destination cassette of pMW138-1 and lower-case letters correspond to 50 bp regions homologous to PAP85. | |
Rozwadowski et al. BMC Biotechnology 2008 8:88 doi:10.1186/1472-6750-8-88 |
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