Table 2

Labelled cDNA yield starting from 20 μg of mouse total RNA

HyPer5 NHS

Cy5 NHS

HyPer5-dCTP

Cy5-dCTP


Dye incorporation

87.1

34.5

32

35


Nucleotide per dye ratio*

29

77

94

70


*Nucleotide per dye ratios was determined by [DNA yield × 1000]/[dye incorporation × 324.5]. Data represented is average yield from n = 35 for HyPer5 NHS, n = 33 for Cy5 = NHS, and triplicates for HyPer5- and Cy5-dCTP.

Dar et al. BMC Biotechnology 2008 8:86   doi:10.1186/1472-6750-8-86

Open Data