Spectral absorption of curcuminoids solubilized in butanol. A) Spectral scan of 6 uM curcuminoids in water-saturated 1-butanol (white area) between wavelength 370 and 460 nm with increments of 1 nm. The gray area delineates the background absorption of water-saturated butanol. B) Dose curve of curcuminoids (1–80 μM) solubilized in water-saturated butanol. Data are presented as absorption (OD) as a function of curcuminoid concentration (μM) at a wavelength of 427 nm. C) Elution profile after reversed phase chromatography of 50 μM curcuminoids dissolved in 27.5% acetonitrile solution (see Methods). The vertical bar represents 20 milliabsorption units (mAU). Elution peaks of curcuminoids at indicated retention times (rt) designate bisdemethoxycurcumin (B), demethoxycurcumin (D), and curcumin (C).
Quitschke BMC Biotechnology 2008 8:84 doi:10.1186/1472-6750-8-84