Measuring microRNAs: Comparisons of microarray and quantitative PCR measurements, and of different total RNA prep methods

Additional File 1:

qRT-PCR Ct data and microarray signal data for 61 miRNAs. Mean Ct values data for the four qPCR replicates are listed, as are mean microarray data (log₂ of the total gene signal) for the microarray replicates.

Format: XLS Size: 78KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional File 2:

Comparison of qPCR and microarray miRNA profiling for individual miRNAs. Scatter plots for 51 miRNAs not shown in Figure 1.

Format: PDF Size: 176KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 3:

Comparison of qPCR and microarray miRNA profiling for individual miRNAs. Scatter plots for 51 miRNAs not shown in Figure 1.

Format: PDF Size: 179KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 4:

Comparison of qPCR and microarray miRNA profiling for individual miRNAs. Scatter plots for 51 miRNAs not shown in Figure 1.

Format: PDF Size: 173KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 5:

Comparison of qPCR and microarray miRNA profiling for individual miRNAs. Scatter plots for 51 miRNAs not shown in Figure 1.

Format: PDF Size: 174KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 6:

Comparison of qPCR and microarray miRNA profiling for individual miRNAs. Scatter plots for 51 miRNAs not shown in Figure 1.

Format: PDF Size: 173KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 7:

Comparison of qPCR and microarray miRNA profiling for individual miRNAs. Scatter plots for 51 miRNAs not shown in Figure 1.

Format: PDF Size: 122KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 8:

Comparison of qPCR and microarray miRNA profiling for 60 miRNAs in tissue pairs. Scatter plots for 32 tissue pairs not shown in Figure 3.

Format: PDF Size: 112KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 9:

Comparison of qPCR and microarray miRNA profiling for 60 miRNAs in tissue pairs. Scatter plots for 32 tissue pairs not shown in Figure 3.

Format: PDF Size: 112KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 10:

Comparison of qPCR and microarray miRNA profiling for 60 miRNAs in tissue pairs. Scatter plots for 32 tissue pairs not shown in Figure 3.

Format: PDF Size: 112KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 11:

Comparison of qPCR and microarray miRNA profiling for 60 miRNAs in tissue pairs. Scatter plots for 32 tissue pairs not shown in Figure 3.

Format: PDF Size: 111KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 12:

Comparison of qPCR and microarray miRNA profiling for 60 miRNAs in tissue pairs. Scatter plots for 32 tissue pairs not shown in Figure 3.

Format: PDF Size: 110KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 13:

Comparison of qPCR and microarray miRNA profiling for 60 miRNAs in tissue pairs. Scatter plots for 32 tissue pairs not shown in Figure 3.

Format: PDF Size: 113KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 14:

Comparison of qPCR and microarray miRNA profiling for 60 miRNAs in tissue pairs. Scatter plots for 32 tissue pairs not shown in Figure 3.

Format: PDF Size: 114KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 15:

Comparison of qPCR and microarray miRNA profiling for 60 miRNAs in tissue pairs. Scatter plots for 32 tissue pairs not shown in Figure 3.

Format: PDF Size: 112KB Download file

This file can be viewed with: Adobe Acrobat Reader

Additional File 16:

List of microarray hybridizations done with HeLa and ZR-75-1 preps. Three or four preps done with each method (labeled A-D) were hybridized for the indicated number of times.

Format: XLS Size: 22KB Download file

This file can be viewed with: Microsoft Excel Viewer

Additional File 17:

List of miRNAs measured at higher levels in one prep type over another. miRNAs found at levels either > 2x or between 1.5x and 2x in one prep type over another, in HeLa and ZR-75-1 total RNA preps. Ratios are the ratio of normalized total gene signals on microarrays in prep 1 versus prep 2.

Format: XLS Size: 24KB Download file

This file can be viewed with: Microsoft Excel Viewer