The effect of the size-separated fractions on in vitro protein synthesis. A. Screening the fractions (10 to 21) from a size-exclusion separation of an S30 extract for enhancement in in vitro protein synthesis in the PURE system. The activities were assayed at 60 min. for Fluc (grey bars) and β-galactosidase (black bars), and by comparing to the activities of the controls (supplemented with only the column buffer), the data are presented as "fold increase". B. Comparison of the protein synthesis yields of Fluc between the PURE system (PURE) without any supplement (Buffer) or supplemented with the fraction 17 (F17) or the S30 extract (Sup), with the S30 systems from Invitrogen (Expressway) and Roche (RTS). The yields were calculated from the activities of the in vitro synthesis reactions compared to that of the known amount of pure Fluc protein assayed at the same time.
Hillebrecht and Chong BMC Biotechnology 2008 8:58 doi:10.1186/1472-6750-8-58