Table 5

NA6 qPCR analysis of E2 and E6 copy numbers in cervical SCC samples.

Cervical SCC sample ID

E2 copies

E6 copies

Viral load level

Ratio of E2/E6


G30

2

1

Low

1.28

G1

0

2

Low

0.21

G31

0

4

Low

0.06*

n55

0

5

Low

0.01*

G19

12

10

Low

1.26

G12

35

35

Low

0.99

n12

44

42

Low

1.03

n10

73

48

Low

1.54


G26

461

297

Medium

1.55

G18

258

343

Medium

0.75

G6

467

423

Medium

1.10

G3

384

528

Medium

0.73

G9

0

691

Medium

0.00*


G11

954

2,481

High

0.38


HPV gene copy numbers are per diploid genome. E2/E6 ratio values were calculated from the qPCR readings for E2 and E6 and rounded to the nearest two decimal places. The only samples in which HPV16 physical state could be inferred with confidence are marked by asterisks in the right hand column.

Roberts et al. BMC Biotechnology 2008 8:57   doi:10.1186/1472-6750-8-57

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