Figure 3.

In vitro binding and inhibition of BChE/hSA fusion protein contained in the milk of transgenic mice by OP nerve agents. Binding and inhibition were performed with diluted raw mouse milk materials from a F1 transgenic mouse, 307-1A7 (A) and a control FVB mouse (B, 0.086 ± 0.03 unit/ml). The reactions were carried out in the presence of tabun (GA, ◆), sarin (GB, ■), soman (GD, ▲) and VX (●), respectively. Residual BChE activity was measured by the Ellman assay. Data points represent the mean ± SD from duplicates in each mouse.