Figure 5.

Comparison of actual reaction fluorescence readings to those predicted by LRE modeling. Although the DyNAmo series was selected for presentation, all three series presented in Figure 2 produced similar results. The average F₀ calculated from the LRE window (designated by red circles, see Figure 3) was used to calculate the predicted F_C across the entire amplification profile using equation 7 (circles) and plotted with the actual F_C readings (dots). The numerical summaries presented as inlays illustrate the high degree of precision that can be achieved, with the average percent difference of < 0.5% for F_C readings within the LRE window. F_C: actual reaction fluorescence, pF_C: predicted reaction fluorescence, Diff: difference between actual and predicted F_C, %pF_C: difference expressed as a percentage of the predicted F_C.