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Open Access Highly Accessed Methodology article

A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR

Robert G Rutledge* and Don Stewart

BMC Biotechnology 2008, 8:47  doi:10.1186/1472-6750-8-47

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LRE qPCR Website

Bob Rutledge   (2014-11-20 11:10)  Natural Resources Canada email

Please note that a website has been constructed that provides additional information about LRE qPCR, in addition to providing the most current version of the LRE Analyzer, a fully featured Java desktop program that automates LRE-based quantification.

https://sites.google.com/site/lreqpcr/

The site also includes a three part video that provides a detailed overview of how LRE qPCR was developed and tested.

https://sites.google.com/site/lreqpcr/lre-overview

Competing interests

I have no competing interests.

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Updated version for the Java program

Bob Rutledge   (2009-10-15 10:32)  Canadian Forest Service email

The prototypic Java program provided as supplementary materials has been updated, which fixes a number of bugs and provides a somewhat improved algorithm for LRE window selection. A copy of this updated program can be obtained by request to the corresponding author.

Competing interests

None declared

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