Figure 5.

Analysis of ConA purified glycoproteins in WT and patatin-hpRNAi lines. (A) Determination of total soluble proteins and ConA purified glycoproteins by Bradford assay. One gram of fresh weight of tuber was used for the extraction of total soluble proteins and purification of glycoprotein. (B) SDS-PAGE analysis of ConA purified glycoproteins in WT and patatin-hpRNAi line 4 and 8. Extract (total soluble proteins), ConA-nonbinding and ConA-binding fractions were separated on 10% SDS-PAGE gel and stained with coomassie blue. Patatin was found to be approximately 40 kDa in size in wild-type plants only. Lane M, molecular mass marker. 20 μg of each fraction was loaded in each well. Marker sizes (in kDa) are indicated.