Glycosyltransferase efficiently controls phenylpropanoid pathway

Anna Aksamit-Stachurska² , Alina Korobczak-Sosna¹ , Anna Kulma¹ and Jan Szopa¹

¹Faculty of Biotechnology, Wroclaw University, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland

²Faculty of Biological Sciences, Wroclaw University, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland

author email corresponding author email

BMC Biotechnology 2008, 8:25doi:10.1186/1472-6750-8-25


Published:	5 March 2008

Abstract

Background

In a previous study, anthocyanin levels in potato plants were increased by manipulating genes connected with the flavonoid biosynthesis pathway. However, starch content and tuber yield were dramatically reduced in the transgenic plants, which over-expressed dihydroflavonol reductase (DFR).

Results

Transgenic plants over-expressing dihydroflavonol reductase (DFR) were subsequently transformed with the cDNA coding for the glycosyltransferase (UGT) of Solanum sogarandinum in order to obtain plants with a high anthocyanin content without reducing tuber yield and quality. Based on enzyme studies, the recombinant UGT is a 7-O-glycosyltransferase whose natural substrates include both anthocyanidins and flavonols such as kaempferol and quercetin. In the super-transformed plants, tuber production was much higher than in the original transgenic plants bearing only the transgene coding for DFR, and was almost the same as in the control plants. The anthocyanin level was lower than in the initial plants, but still higher than in the control plants. Unexpectedly, the super-transformed plants also produced large amounts of kaempferol, chlorogenic acid, isochlorogenic acid, sinapic acid and proanthocyanins.

Conclusion

In plants over-expressing both the transgene for DFR and the transgene for UGT, the synthesis of phenolic acids was diverted away from the anthocyanin branch. This represents a novel approach to manipulating phenolic acids synthesis in plants.