Figure 4.

Representative results of serum p53 autoantibodies of lung cancer patients detected by immunoblotting. PVDF membrane containing 1 μg/strip of the purified (His)6-p53 fusion protein was incubated with 1:5,000 p53 mAb DO7 as positive control, 1:200 diluted sera from lung cancer patients, respectively. Lane PC, positive control: probing with p53 mAb DO7, lane A-I, probing with serum from lung cancer patients.