Figure 5.

Analysis of transgene expression by hMSC-NT3-EGFP cell transplants following laser capture micro-dissection (LCM). (A) Laser Capture Micro-dissection (LCM) of hMSC-NT3-EGFP cell transplants at day 1 and day 7 post-transplantation in rat spinal cord. Pictures showing cresyl-violet stained slides pre-and post-LCM. (B) Upper graph showing the level of transgenic EGFP mRNA transcripts/1000 copies GAPDH in hMSC-EGFP cell cultures (CELLS, n = 1), on transplanted hMSC-EGFP cells at day 1 post-transplantation (DAY 1, n = 1), and on transplanted hMSC-EGFP cells at day 7 post-transplantation (DAY 7, n = 1). Lower graph showing the level of transgenic EGFP mRNA transcripts/1000 copies GAPDH in hMSC-NT3-EGFP cell cultures (CELLS, n = 1), on transplanted hMSC-NT3-EGFP cells at day 1 post-transplantation (DAY 1, n = 1), and on transplanted hMSC-NT3-EGFP cells at day 7 post-transplantation (DAY 7, n = 1).