|
Monosaccharide composition of the N-linked oligosaccharide core structure of IgG1 from clones transformed with the FUT8 and GMD siRNA tandem expression vector during fed-batch culture |
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| Clone Name |
Culture period (day) |
Relative composition of monosaccharide |
Fucose(-)%b |
||
|
|
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| Fucosea |
GlcNAc |
Mannose |
|||
|
|
|||||
| 32-05-12 |
6 |
0.92 |
4.00 |
2.86 |
8 |
| 12 |
0.94 |
4.00 |
2.99 |
6 |
|
| 16 |
0.97 |
4.00 |
3.01 |
3 |
|
| FG1 |
6 |
n.d. |
4.00 |
2.85 |
100 |
| 12 |
n.d. |
4.00 |
2.97 |
100 |
|
| 16 |
n.d. |
4.00 |
2.92 |
100 |
|
| FG16 |
6 |
n.d. |
4.00 |
2.82 |
100 |
| 12 |
n.d. |
4.00 |
2.96 |
100 |
|
| 16 |
n.d. |
4.00 |
2.96 |
100 |
|
|
aMolar ratios calculated vs. 4 GlcNAc; n.d., not detectable. bTotal percentage of non-fucosylated Fc oligosaccharides calculated by the formula (1-a) × 100. | |||||
Imai-Nishiya et al. BMC Biotechnology 2007 7:84 doi:10.1186/1472-6750-7-84 |
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