Table 2

Monosaccharide composition of the N-linked oligosaccharide core structure of IgG1 from clones transformed with siRNA expression vectors
Clone Name
 siRNAa
 LFb
 Relative composition of monosaccharide
 Fucose(-)%d







Fucosec
 GlcNAc
 Mannose
32-05-12
 -
 -
 0.97
 4.00
 2.67
 3
GMDb2
 GMD
 -
 0.22
 4.00
 2.52
 78
GMDb5
 GMD
 -
 0.21
 4.00
 2.53
 79
GMDb2-F3
 GMD·FUT8
 -
 0.02
 4.00
 2.70
 98
GMDb2-F5
 GMD·FUT8
 -
 0.03
 4.00
 2.71
 97
GMDb5-F2
 GMD·FUT8
 -
 0.05
 4.00
 2.77
 95
GMDb5-F4
 GMD·FUT8
 -
 0.09
 4.00
 2.70
 91
32-05-12
 -
 +
 0.94
 4.00
 2.56
 6
GMDb2
 GMD
 +
 0.94
 4.00
 2.56
 6
GMDb5
 GMD
 +
 0.92
 4.00
 2.60
 8
GMDb2-F3
 GMD·FUT8
 +
 0.19
 4.00
 2.52
 81
GMDb2-F5
 GMD·FUT8
 +
 0.21
 4.00
 2.54
 79
GMDb5-F2
 GMD·FUT8
 +
 0.19
 4.00
 2.55
 81
GMDb5-F4
 GMD·FUT8
 +
 0.33
 4.00
 2.68
 67

aGMD, introduction of GMD siRNA expression vector; GMD·FUT8, introduction of FUT8 siRNA expression vector to GMD siRNA-introduced cells.

bLF, addition of 100 μM L-fucose into the culture medium.

cMolar ratios calculated vs. 4 GlcNAc.

dTotal percentage of non-fucosylated Fc oligosaccharides calculated by the formula (1-c) × 100.

Imai-Nishiya et al. BMC Biotechnology 2007 7:84   doi:10.1186/1472-6750-7-84