Table 1 |
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|
Effects of LOWESS Normalization on Correlations. |
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|
Effects of LOWESS Normalization |
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|
|
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|
Scan Interval (minutes): |
0 |
13 |
26 |
39 |
52 |
65 |
78 |
91 |
|
|
|
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|
Non- carbon-filtered |
Raw ratio data |
1.00 |
0.93 |
0.81 |
0.69 |
0.59 |
0.48 |
0.40 |
0.37 |
|
Normalized ratio data |
1.00 |
0.98 |
0.96 |
0.94 |
0.92 |
0.88 |
0.84 |
0.80 |
|
|
Cy5 Intensity data |
1.00 |
0.98 |
0.96 |
0.94 |
0.90 |
0.85 |
0.80 |
0.76 |
|
|
Cy3 Intensity data |
1.00 |
0.99 |
0.99 |
0.99 |
0.98 |
0.98 |
0.98 |
0.97 |
|
|
|
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|
Carbon-filtered |
Raw ratio data |
1.00 |
0.99 |
0.99 |
0.99 |
0.98 |
0.98 |
0.97 |
0.96 |
|
Normalized ratio data |
1.00 |
0.99 |
0.99 |
0.99 |
0.98 |
0.98 |
0.98 |
0.98 |
|
|
Cy5 Intensity data |
1.00 |
0.99 |
0.99 |
0.99 |
0.99 |
0.99 |
0.99 |
0.99 |
|
|
Cy3 Intensity data |
1.00 |
0.99 |
0.99 |
0.99 |
0.99 |
0.99 |
0.99 |
0.99 |
|
|
|
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|
Intensity data and Cy5/Cy3 ratio (raw) data were obtained for individual microarray features at each scan time using the Axon GenePix Pro software. LOWESS normalization was performed on the ratio data. The data shown are correlations (r-values) of the raw or normalized data at each scan interval compared to the 0-minute scan interval. |
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Branham et al. BMC Biotechnology 2007 7:8 doi:10.1186/1472-6750-7-8 |
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