Heating as a rapid purification method for recovering correctly-folded thermotolerant VH and VHH domains

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Methodology article

Aurelien Olichon¹ , Daniel Schweizer² , Serge Muyldermans³ and Ario de Marco²^,4

¹Cell Biology Unit, EMBL – Meyerhofstr. 1, D-69117, Heidelberg, Germany

²Scientific Core Facilities, EMBL – Meyerhofstr. 1, D-69117, Heidelberg, Germany

³Department of Cellular and Molecular Interactions, VIB, Vrije Unversiteit Brussel, Pleinlaan 2, B-1050, Brussel, Belgium

⁴IFOM-IEO Campus, Biochemistry Unit, via Adamello 16, I-20139, Milano, Italy

author email corresponding author email

BMC Biotechnology 2007, 7:7doi:10.1186/1472-6750-7-7


Published:	26 January 2007

Additional File 1:

Seq_VHHs. A PDF File containing the sequences of the 5 VHHs used for the heat-dependent purification experiment.

Format: PDF Size: 14KB Download file

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