Table 2 |
||||||
|
Proteins not expressing in E. coli in vitro or in wheat germ or in both systems. Molecular weight was calculated by translation of the DNA sequence. Localization information was taken from the Uniprot database. Domain information was retrieved from the Pfam database: cc: coiled coil, tms: transmembrane segment, sp signal peptide. Empty fields correspond to no assignment in the database. |
||||||
|
Hit Acc. No. |
Gene symbol |
Mw in kDa |
Localization |
Domains |
Expression in E. coli in vitro |
Expression in wheat germ |
|
|
||||||
|
NM_007317 |
KIF22 |
74 |
nuclear |
1cc,, |
no |
no |
|
NM_015723 |
PNPLA8 |
89 |
membrane |
no |
no |
|
|
NM_020748 |
KIAA1287 |
135 |
n/a |
no |
no |
|
|
NM_018127 |
ELAC2 |
93 |
nuclear |
no |
no |
|
|
NM_015072 |
TTLL5 |
92 |
n/a |
3cc,, |
no |
no |
|
NM_014585 |
SLC40A1 |
63 |
membrane |
,,10tms |
no |
no |
|
NM_020470 |
YIF1A |
32 |
membrane |
,,5tms |
no |
yes |
|
NM_004394 |
DAP |
12 |
secreted |
no |
yes |
|
|
NM_001014835 |
PAK4 |
64 |
n/a |
no |
yes |
|
|
NM_013296 |
GPSM2 |
55 |
n/a |
no |
yes |
|
|
NM_006812 |
OS-9 |
70 |
n/a |
1cc1sp1tms |
no |
yes |
|
NM_014860 |
SUPT7L |
47 |
n/a |
1cc,, |
no |
yes |
|
NM_003908 |
EIF2S2 |
39 |
nuclear |
no |
yes |
|
|
NM_006321 |
ARIH2 |
58 |
nucleus |
2cc,, |
no |
yes |
|
NM_002816 |
PSMD12 |
53 |
cytosol |
1cc,, |
no |
yes |
|
NM_000984 |
RPL23A |
18 |
cytosol |
no |
yes |
|
|
NM_002134 |
HMOX2 |
36 |
microsomal |
1cc,1tms |
yes |
no |
|
NM_018099 |
MLSTD1 |
60 |
intracellular |
,,2tms |
yes |
no |
|
NM_013277 |
RACGAP1 |
71 |
intracellular |
1cc,, |
yes |
no |
|
NM_003263 |
TLR1 |
91 |
membrane |
1sp,1tms |
yes |
no |
|
NM_032292 |
FLJ20203 |
91 |
n/a |
1cc,, |
yes |
no |
|
NM_014149 |
HSPC049 |
78 |
n/a |
1cc,, |
yes |
no |
|
|
||||||
|
Langlais et al. BMC Biotechnology 2007 7:64 doi:10.1186/1472-6750-7-64 |
||||||
