Table 1 |
||||
|
Primers and probe used in this study |
||||
|
Primer/probe |
Target/specificity |
Sequence (5'-3') |
Product size (bp) |
Reference |
|
|
||||
|
Bak11w |
16S rDNA |
AGT TTG ATC MTG GCT CAG |
[35] |
|
|
Bak4 |
AGG AGG TGA TCC ARC CGC A |
[36] |
||
|
P1 |
pedA-pedB |
AAA ATA TCT AAC TAA TAC TTG |
||
|
P2 |
TAA AAA GAT ATT TGA CCA AAA |
711 |
[23] |
|
|
pedopF |
pedB-pedD |
GGG CGA GTT TAA CAT GCT AGA |
||
|
pedopR |
TGA TTA TGA ATT AAC CGT GCA |
2862 |
This study |
|
|
pedseqA |
Sequencing of the pediocin operon |
CTT GCT CGA TAA TGG TAA |
||
|
pedseqB |
CTA TCA GGT AAC TGA AAA |
|||
|
pedseqC |
CAC GCT TTT CTG ATG CAA |
|||
|
pedseqD |
TTC TTG ACC CCA TTA GAA |
This study |
||
|
pedseqL |
Upstream of operon |
AGC AAT TAC AGT CAA CCA TAA CCA T |
||
|
pedseqH |
ACA GAG CAG GAA TGT TTG CCA CAA G |
1009 |
This study |
|
|
pedseq M |
Downstream of operon |
ATT GTC TCC GGC ACA ATG TT |
||
|
pedseqN |
CAA AGT GGG GAA ACT CGA AA |
1417 |
This study |
|
|
pedA2RTF |
Real-time PCR |
GGC CAA TAT CAT TGG TGG TA |
||
|
pedA2RTR |
ATT GAT TAT GCA AGT GGT AGC C |
|||
|
TqM-pedA |
FAM-ACT TGT GGC AAA CAT TCC TGC TCT GTT GA-TAMRA |
100 |
This study |
|
|
|
||||
|
Mathys et al. BMC Biotechnology 2007 7:55 doi:10.1186/1472-6750-7-55 |
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