Table 1 |
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Primers. List of oligonucleotides used for vector construction and Fc region cloning. |
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|
Number |
Name |
Orientation |
Sequence |
|
|
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|
A |
Hygro-XbaI-inv |
Antisense |
AGCTTCTAGACAGCTTGGGTCTCCCTATAG |
|
B |
Hygro-HindIII-inv |
Sense |
AGCTAAGCTTAAACCCGCTGATCAGC |
|
1 |
1-XbaI-leader |
Sense |
CAGGCGTCTAGATGCCACCATGGGCTGGAGCCTGATCCTCCTGTTCCTCGTCGCTGTGGCTACAGGTAAGGG |
|
2 |
2-leader-intron |
Sense |
TGTGGCTACAGGTAAGGGGCTCACAGTAGCAGGCTTGAGGTCTGGACATATATATGGGTGACAATGACATCCAC |
|
3 |
3-BssHII-scFv |
Sense |
GGTGACAATGACATCCACTTTGCCTTTCTCTCCACAGGTGGCGCGCATGCCGACATCCGGTTGACCCAG |
|
4 |
scFv-NheI-HindIII |
Antisense |
CCGCTAAGCTTCGCCTGGCTAGCAAAAGCGTCCGTCGTATC |
|
5 |
HuGCH2-s |
Sense |
AGGCGGCTAGCGACAAAACTCACACATGCCCACCGTGCCCA |
|
6 |
HuGCH3-SV5-a |
Antisense |
CTGCTAAGCTTTTAAGTACTATCCAGGCCCAGCAGTGGGTTTGGGATTGGTTTGCCACTAGTTTTACCCGGGGACAGGGAGAG |
|
7 |
HuACH2-s |
Sense |
CAGGCGGCT AGCGTTCCCTCAACTCCACCTACC |
|
8 |
HuACH3-a |
Antisense |
CCGCTACTAGTTTTACCCGCCAAGCGGTCGAT |
|
9 |
MoGCH3-a |
Antisense |
CCGCTACTAGTTTTACCAGGAGAGTGGGAGAG |
|
10 |
MoGCH2-s |
Sense |
CAGGCG GCT AGC GGTTGTAAGCCTTGCATATGTACA |
|
11 |
RaGCH3-a |
Antisense |
CCGCTACTAGTTTTACCCGGAGGCCGGGAGATG |
|
12 |
RaGCH2-s |
Sense |
CAGGCG GCT AGC CACAAATGCCCTACATGCCCT |
|
|
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Di Niro et al. BMC Biotechnology 2007 7:46 doi:10.1186/1472-6750-7-46 |
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