|
Spectral characteristics of GCaMP-like Ca2+ sensors. Excitation/emission wavelengths, fluorescence quantum yield, extinction coefficient and pH stability in the presence of Ca2+are shown |
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| Sensor |
(F/F0)max |
Excitation/emission wavelengths, nm |
Quantum yield |
Extinction coefficient, M-1cm-1 |
Relative brightness a |
pH stability (pKa) |
Kd for Ca2+, μM |
|
|
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| Flash -pericam |
8.0 |
494/514 |
0.20 |
16,900 |
0.10 |
7.9 |
0.7 |
| GCaMP1 |
4.3 |
488/510 |
0.05 |
1,400 |
0.002 |
7.1 |
0.24 |
| GCaMP1.6 |
4.9 |
488/509 |
0.79 |
3,800 |
0.09 |
8.2 |
0.16 |
| GCaMP2 |
5.0 |
487/508 |
0.93 |
19,000 |
0.53 |
Nd |
0.16 |
| Case12 |
12.0 |
491/516 |
0.24 |
48,000 |
0.35 |
7.2 |
1.0 |
| Case16 |
16.5 |
490/516 |
0.17 |
50,000 |
0.28 |
7.2 |
1.0 |
|
a Calculated brightness compared to that of EGFP. | |||||||
Souslova et al. BMC Biotechnology 2007 7:37 doi:10.1186/1472-6750-7-37 |
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