Figure 3.

Construction of HSV2-BAC ΔgD. (A) Visualization of EGFP-containing BAC colonies after transformation following the allele replacement procedure. Bacterial clones harboring mutant (ΔgD) HSV2-BAC glow green because of insertion of EGFP at the site of US6. (B) Schematic diagram of HSV2-BAC with EGFP insertion at US6(gD). pBeloBAC11 refers to the insertion of the BAC sequences into UL23 (tk) of the viral genome.