Table 1

DNA transformation efficiency of L. lactis spp. lactis cells with and without pretreatment with LiAc and DTT. Results are the mean (± SD) of 6 replications

LiAc (mM)

DTT (mM)

Transformants/μg DNA

L. lactis LM0230

L. lactis ATCC 11454


0

0

1.20 ± 0.50 × 105

1.00 ± 0.48 × 104

100

0

1.10 ± 0.52 × 105

1.20 ± 0.50 × 104

0

10

13.00 ± 8.02 × 106

7.00 ± 5.01 × 105

100

10

225.00 ± 52.51 × 107

117.00 ± 42.55 × 106


Papagianni et al. BMC Biotechnology 2007 7:15   doi:10.1186/1472-6750-7-15

Open Data