Confocal microscopy analysis of intracellular distribution of TAT-SA or SA at 4 h post transduction or injection. (A-C) Human cancer cell lines (HeLa, A549) and a non-cancer cell line (MRC-5) were transduced with TAT-SA prior to PFA fixation, permeabilization with Triton-X and immunolabeling with rabbit SA Ab followed by Alexa-488-conjugated goat anti-rabbit IgG (green). (D-E) Living HeLa cells were transduced or (F) injected to the cytoplasm with TAT-SA-A488 (green), or (G-H) transduced or (I) injected with SA-A488 (green). Scale bars, 10 μm.
Rinne et al. BMC Biotechnology 2007 7:1 doi:10.1186/1472-6750-7-1