Open Access Research article

Internalization of novel non-viral vector TAT-streptavidin into human cells

Johanna Rinne1, Brian Albarran2, Juulia Jylhävä1, Teemu O Ihalainen1, Pasi Kankaanpää1, Vesa P Hytönen13, Patrick S Stayton2, Markku S Kulomaa14 and Maija Vihinen-Ranta1*

Author Affiliations

1 NanoScience Center; Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland

2 Department of Bioengineering, University of Washington, Seattle, WA, USA

3 Department of Materials, ETH Zürich, Zürich, Switzerland

4 Institute of Medical Technology, University of Tampere, Tampere, Finland

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BMC Biotechnology 2007, 7:1  doi:10.1186/1472-6750-7-1

Published: 2 January 2007

Additional files

Additional file 1:

Characterization of TAT-SA constructs by SDS-PAGE and Western Blot Analysis. TAT-SA (lanes 1, 3) and TAT-SA bound to biotin (lanes 2, 4) are shown as tetrameric conformations (60 kD) in reducing conditions mimicking the cellular environment at 22°C or after preheatment to 37°C.

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Open Data

Additional File 2:

The role of macropinocytosis in the internalization of TAT-SA in living HeLa cells. Three-dimensional (3D) illustration of colocalization of TAT-SA-A488 (green) and macropinocytosis marker TRITC-labeled dextran (10 kD, red) at 15 min post transduction by confocal microscopy. Merged 3D images of TAT-SA-A488 and TRITC-dextran together (yellow) with DIC images were used for construction of the QuickTime movie.

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Additional file 3:

The cytoplasmic distribution of TAT-SA-biotin. Three-dimensional (3D) illustration of localization of TAT-SA-A488 (green) complexed with Biotin-DY-633 (purple) in the living cells at 4 h post transduction (colocalization, white) by confocal microscopy. The merged 3D images were used to construct the QuickTime movie. The close-up box shows the magnified 3D projection slice of the nuclear area.

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Open Data

Additional file 4:

The cytoplasmic and nuclear distribution of TAT-SA in the presence of biotinylated PPAA polymer. Three-dimensional (3D) illustration of localization of TAT-SA-A488 (green) complexed with biotinylated endosomal-releasing peptide PPAA in the living cells at 4 h post transduction by confocal microscopy. The merged 3D images of the different structures were used to construct the QuickTime movie. The close-up box shows the magnified 3D projection slice of the nuclear area.

Format: MPG Size: 5.9MB Download file

Playing the movie within this page requires QuickTime 6.4 or later and JavaScript. Read more

Open Data