Figure 7.

Recombinant DI in solution is an effective inhibitor of a pathogenic monoclonal human aPL in vitro. Competitive inhibition of affinity purified IS4V_H/IS4V_L binding to his₆tagged-DI bound to a nickel chelate plate, by recombinant DI and wild type β₂GPI as inhibitors. A constant amount of antibody was mixed with varying concentrations of inhibitor and allowed to equilibrate at room temperature. For a given μmol concentration of inhibitor, DI alone is a more effective inhibitor of IS4V_H/IS4V_L than whole β₂GPI.