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Resolution: standard / high Figure 6.
Recombinant DI binds monoclonal human aPL with the same order of binding as seen with whole β2GPI. ELISA of β2GPI (fig. 6a) and his6-tagged DI (fig. 6b) binding to IS4VH/B3VL. IS4VH/IS4VL and IS4VH/UK-4VL. The same order of binding of aPL to both β2GPI and DI was observed. Greatest binding was seen with IS4VH/B3VL and least with IS4VH/UK-4VL.
Ioannou et al. BMC Biotechnology 2006 6:8 doi:10.1186/1472-6750-6-8 |