Multiplexed expression and screening for recombinant protein production in mammalian cells
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* Corresponding author: Michael R Dyson mrd@sanger.ac.uk
BMC Biotechnology 2006, 6:49 doi:10.1186/1472-6750-6-49
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Commentary
Towards universal systems for recombinant gene expression Hans Sørensen Microbial Cell Factories 2010, 9:27 (30 April 2010) |
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Method
Application of phage display to high throughput antibody generation and characterization Darren J Schofield, Anthony R Pope, Veronica Clementel, Jenny Buckell, Susan DJ Chapple, Kay F Clarke, Jennie S Conquer, Anna M Crofts, Sandra RE Crowther, Michael R Dyson, Gillian Flack, Gareth J Griffin, Yvette Hooks, William J Howat, Anja Kolb-Kokocinski, Susan Kunze, Cecile D Martin, Gareth L Maslen, Joanne N Mitchell, Maureen O'Sullivan, Rajika L Perera, Wendy Roake, S Paul Shadbolt, Karen J Vincent, Anthony Warford, Wendy E Wilson, Jane Xie, Joyce L Young, John McCafferty Genome Biology 2007, 8:R254 (29 November 2007) A phage display library has been constructed containing over 1010 human antibodies, allowing the large-scale generation of antibodies. Over 38,000 recombinant antibodies against 292 antigens were selected, screened and sequenced, and 4,400 resultant unique clones characterized further. |