Table 1

Primer List

pET28 T7 replace PCR

CCTATAGTGAGTCGTATTAATTTCGCGGGATCG

pET28 lacO PCR

phos-GGAATTGTGAGCGGATAACAATTCCCCTC

5' Amtr pro Himar1

GTTGTTAGATCTGGCTCCTCCTAGAACGATCGC

3' Amtr pro Himar1

phos-GATAACTTAGGATAAATCATACATAATGTTAATGCAACAG

5' Am tr pro

GTTGTTGAATTCGGCTCCTCCTAGAACGATCGC

3' Am tr pro

GTTGTTGGATCCTATCCCTTATGTTACTCAGATAACTTAGGATAAATCA

GFPuv SDM A

CCACAACATTGAAGATGGTTCCGTTCAACTAGCAGACC

GFPuv SDM B

GGTCTGCTAGTTGAACGGAACCATCTTCAATGTTGTGG

5' GFPuv PCR

GTTGTTGGATCCATGAGTAAAGGAGAAGAACTTTTCACTG

3' GFPuv PCR phos

phos-AATCCCTATTTGTAGAGCTCATCCATGCCATGTGTAATC

3' S-S Xba PCR

GTTGTTTCTAGATTATTTGCCGACTACCTTGGTGATCTCGCCTTTC

5' S-S PCR phos

phos-AAATTATGAGGGAAGCGGTGATCGCCGAAGTATCGACTC

Bam re SDM A

CTGAGTAACATAAGGGATATTATAATGAGTAAAGGAGAAGAAC

Bam re SDM B

GTTCTTCTCCTTTACTCATTATAATATCCCTTATGTTACTCAG

Himar1 right repeatA

TGTTGAAGCTTAGACCGGGGACTTATCAGCCAACCTGTTACTCGAGTTGT

Himar1 right repeatB

ACAACTCGAGTAACAGGTTGGCTGATAAGTCCCCGGTCTAAGCTTCAACA

Himar1 left repeatA

TGTTGAGATCTAACAGGTTGGCTGATAAGTCCCCGGTCTGAATTCTTGTT

Himar1 left repeatB

AACAAGAATTCAGACCGGGGACTTATCAGCCAACCTGTTAGATCTCAACA

UV-SS up and out

CATCACCTTCACCCTCTCCACTGAC

UV-SS down and out

CAGCCCGTCATACTTGAAGCTAGGC

Forward UV-SS confirmation PCR

AAAGATGACGGGAACTACAAGACG

Reverse UV-SS confirmation PCR

CACAACAATGGTGACTTCTACAGCG

Felsheim et al. BMC Biotechnology 2006 6:42   doi:10.1186/1472-6750-6-42