Figure 3.

Physical maps of the Himar1 transposon and transposase plasmids. (A) pHIMAR1-UV-SS carries the A. marginale promoter tr driving expression of GFPuv and spectinomycin resistance between the left and right Himar transposon repeats. (B) pET28AMTR-A7-HIMAR contains the A7 hyperactive mutant of the Himar1 transposase also driven by the Am tr promoter.