Influence of the DNA extraction method on PCR efficiency. (A) Variability of PCR efficiency for different DNA isolation methods. Outlier for the CTAB procedure with proteinase K and RNase A treatment is shown as circle above the boxplot. (GS = GENESpin, CTAB.K = CTAB procedure with proteinase K and RNase A treatment). (B) The distribution of PCR efficiencies of 4 tested amplicons on different DNA extracts is presented in boxplots (efficiency data for DNA isolated with Wizard method was excluded because of high variability of results).
Cankar et al. BMC Biotechnology 2006 6:37 doi:10.1186/1472-6750-6-37