Table 1 |
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|
Primer sequences. PVX and epitope-specific primers, including primer sequences and amplification product characteristics for PVX6His2ACP (A), PVX6HisE212ACP (B) and PVX6HisE222ACP (C). |
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|
Name |
Primer sequence 5' to 3' |
Optimal annealing temp. °C |
Amplified fragment size (bp) |
||
|
A |
B |
C |
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|
|
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|
PVX FOR PVX REW |
CTGGGGAATCAATCACAGTG GTCGAATGCAGCGAATTTGTGC |
61.5 |
677 |
890 |
800 |
|
E21 FOR E21REW |
GTCGACGGGCTGTGCCCATTTGATACG GTCGACACTGTTGTGGTCACACAATCC |
63 |
-- |
213 |
-- |
|
E22 FOR E22REW |
GTCGACATGGATTGTGTGACCACAACA GTCGACTACTAGCCCCCCCGTGTAGAC |
64 |
-- |
-- |
123 |
|
|
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|
Marconi et al. BMC Biotechnology 2006 6:29 doi:10.1186/1472-6750-6-29 |
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