Figure 6.

Sera from immunized rabbits contain anti-E2-specific antibodies. Pre-immune sera (A and E) and sera from rabbit immunized with PVX6HisE2¹2ACP (B, C, D) and with PVX6His2ACP (F, G, H) were tested by Western blotting for antibody reactivity against E2 (indicated with a bold arrow) protein (lanes A2, B2, C2, D2, E2, F2, G2 and H2). Western blotting was carried out using serial dilutions 1/10 (B and F), 1/100 (C and G), 1/1,000 (D and H) and pre-immune sera 1/100 (A and E). Lanes A1, B1, C1, D1, E1, F1, G1, and H1 represents Standards Molecular weight.