|
ESI-MS analysis of the tryptic peptides derived from the fused-protein |
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| # |
Peptide sequencea) |
m/z exp. |
m/z theor. |
Zb) |
Abs. Errorc) |
|
|
|||||
| 1 |
11T-K33d) |
908.81 |
908.83 |
3 |
0.02 |
| 2 |
40M-K44 |
639.34 |
639.35 |
1 |
0.01 |
| 3 |
45F-K50 |
685.33 |
685.33 |
1 |
0.00 |
| 4 |
56A-R66f) |
442.87 |
442.88 |
3 |
0.01 |
| 5 |
67A-K114 |
1781.52 |
1781.54 |
3 |
0.02 |
| 6 |
101Q-K114d), e) |
807.93 |
807.95 |
2 |
0.02 |
| 7 |
120N-R151f) |
921.14 |
921.17 |
4 |
0.03 |
| 8 |
166S-R173 |
506.23 |
506.23 |
2 |
0.00 |
| 9 |
177I-K181 |
511.31 |
511.28 |
1 |
0.03 |
| 10 |
190Q-R220f) |
1225.20 |
1225.23 |
3 |
0.03 |
| 11 |
221V-R227d) |
456.74 |
456.75 |
2 |
0.01 |
| 12 |
228M-K236d) |
535.24 |
535.25 |
2 |
0.01 |
|
a) The numbering in the sequences of peptides is according to the fused-protein sequence shown in the section Results and discussion. b) Charge state of individual peptides. c) Indicate the absolute mass difference between the theoretical and experimental molecular masses of the detected peptides. d) Peptides sequenced by ESI-MS/MS. e) Peptide originated by the non-specific cleavage of trypsin f) Peptide containing free cysteine. | |||||
Bello-Rivero et al. BMC Biotechnology 2006 6:25 doi:10.1186/1472-6750-6-25 |
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