Figure 3.

Binding assay of rhu ¹²⁵I^-IFN-γ labeled to recombinant chimeric protein using dot blot analysis. 1 μl aliquots of the eluted fractions from the gel filtration chromatography (rows 1, 2, 3, 4, 5, 6) were applied to nitrocellulose membrane strips. The strips were incubated with IFN-γ labeled with ¹²⁵I (35 μCi/μg) in the absence (lane a) and in the presence (lane b) of 100 fold excess of human IFN-γ.