Additional File 2.

Diagrams for shRNA template generation via complementary annealed oligonucleotides or primer extension using Phi29 DNA polymerase. Both the most common shRNA insert construction technique (complementary annealed oligos) and our proposed alternative (primer extension using Phi29) are diagrammatically represented indicating oligo alignments and the features of each oligo.

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McIntyre et al. BMC Biotechnology 2006 6:1   doi:10.1186/1472-6750-6-1